total ulk Search Results


total ulk 1  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc total ulk 1
Total Ulk 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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total ulk  (Cell Signaling Technology Inc)
98
Cell Signaling Technology Inc total ulk
Total Ulk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/total ulk/product/Cell Signaling Technology Inc
Average 98 stars, based on 1 article reviews
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rabbit cell signaling technology total ulk d8h5  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc rabbit cell signaling technology total ulk d8h5
A table depicting the primary and secondary antibodies used either in Western blot or immunocytochemistry including species reactivity and working dilutions.
Rabbit Cell Signaling Technology Total Ulk D8h5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ulk (total) antibody  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc ulk (total) antibody
( A ) U2OS cells subjected to BAP31 knockout via CRISPR-Cas9 system (sgControl and sgBAP31-2) were subjected to TEM. Higher magnification images are shown at the right. Scale bars, 10 and 1 μm (left and right, respectively). White arrows indicate autolysosomes. ( B ) Total number of autolysosomes in each cell was determined. Data are presented as means ± SD ( n = 6). ( C ) Loss of BAP31 increases LC3-II expression. U2OS cells were transfected with the indicated concentrations of siBAP31 and 150 pmol of siControl for 24 hours. Cells were subjected to immunoblotting using anti-BAP31, anti-LC3, and anti–β-actin antibodies. ( D ) U2OS cells stably expressing GFP-LC3 were transfected with 100 pmol of siBAP31 or siControl for 24 hours. Cells were fixed with 4% paraformaldehyde, and GFP-LC3 (green) fluorescence was determined. Blue represents nuclear 4′,6-diamidino-2-phenylindole (DAPI) staining. Scale bar, 10 μm. The number of LC3-GFP puncta in the cells (green dots) was determined, and data are presented as means ± SD ( n = 6). ( E ) Loss of BAP31 stimulates autophagosome synthesis. U2OS cells were transfected with 100 pmol of siControl or siBAP31 for 24 hours, followed by treatment with or without bafilomycin A1 (1 μg/ml) for 1 hour. Cells were subjected to immunoblotting using the indicated antibodies. ( F ) BAP31 does not affect the ER stress response. U2OS cells were transfected with siBAP31 and siControl for 18 hours and then treated with or without BFA (1 μg/ml) for 8 hours. Cells were subjected to immunoblotting using the indicated antibodies and Phos-tag SDS–polyacrylamide gel electrophoresis (PAGE) or normal SDS-PAGE. ( G ) BAP31 knockout or knockdown activates <t>the</t> <t>AMPK-ULK-LC3</t> signaling pathway. U2OS and HeLa cells subjected to BAP31 knockout via the CRISPR-Cas9 system (sgControl, sgBAP31-2, and sgBAP31-3) and MEF cells transfected with siControl (200 pmol) and siBAP31 at the indicated concentrations for 24 hours were subjected to immunoblotting using the indicated antibodies. P value was calculated using two-way analysis of variance (ANOVA). ** P < 0.01 (B and D).
Ulk (Total) Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
ulk (total) antibody - by Bioz Stars, 2026-03
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total 4ebp1  (Cell Signaling Technology Inc)
98
Cell Signaling Technology Inc total 4ebp1
( A ) U2OS cells subjected to BAP31 knockout via CRISPR-Cas9 system (sgControl and sgBAP31-2) were subjected to TEM. Higher magnification images are shown at the right. Scale bars, 10 and 1 μm (left and right, respectively). White arrows indicate autolysosomes. ( B ) Total number of autolysosomes in each cell was determined. Data are presented as means ± SD ( n = 6). ( C ) Loss of BAP31 increases LC3-II expression. U2OS cells were transfected with the indicated concentrations of siBAP31 and 150 pmol of siControl for 24 hours. Cells were subjected to immunoblotting using anti-BAP31, anti-LC3, and anti–β-actin antibodies. ( D ) U2OS cells stably expressing GFP-LC3 were transfected with 100 pmol of siBAP31 or siControl for 24 hours. Cells were fixed with 4% paraformaldehyde, and GFP-LC3 (green) fluorescence was determined. Blue represents nuclear 4′,6-diamidino-2-phenylindole (DAPI) staining. Scale bar, 10 μm. The number of LC3-GFP puncta in the cells (green dots) was determined, and data are presented as means ± SD ( n = 6). ( E ) Loss of BAP31 stimulates autophagosome synthesis. U2OS cells were transfected with 100 pmol of siControl or siBAP31 for 24 hours, followed by treatment with or without bafilomycin A1 (1 μg/ml) for 1 hour. Cells were subjected to immunoblotting using the indicated antibodies. ( F ) BAP31 does not affect the ER stress response. U2OS cells were transfected with siBAP31 and siControl for 18 hours and then treated with or without BFA (1 μg/ml) for 8 hours. Cells were subjected to immunoblotting using the indicated antibodies and Phos-tag SDS–polyacrylamide gel electrophoresis (PAGE) or normal SDS-PAGE. ( G ) BAP31 knockout or knockdown activates <t>the</t> <t>AMPK-ULK-LC3</t> signaling pathway. U2OS and HeLa cells subjected to BAP31 knockout via the CRISPR-Cas9 system (sgControl, sgBAP31-2, and sgBAP31-3) and MEF cells transfected with siControl (200 pmol) and siBAP31 at the indicated concentrations for 24 hours were subjected to immunoblotting using the indicated antibodies. P value was calculated using two-way analysis of variance (ANOVA). ** P < 0.01 (B and D).
Total 4ebp1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 98 stars, based on 1 article reviews
total 4ebp1 - by Bioz Stars, 2026-03
98/100 stars
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human and mouse phospho ulk1 s556 and total ulk1 elisa  (RayBiotech)
N/A
RayBio Human and Mouse Phospho ULK1 S556 and Total ULK1 ELISA Kit This assay semi quantitatively measures ULK1 phosphorylated at Serine 556 as well as total ULK1 in lysate samples
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pathscan total ulk1 sandwich elisa kit  (Cell Signaling Technology Inc)
N/A
Two related serine threonine kinases UNC 51 like kinase 1 and 2 ULK1 ULK2 were discovered as mammalian homologs of the C elegans gene unc 51 in which mutants exhibited abnormal axonal extension and growth
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A table depicting the primary and secondary antibodies used either in Western blot or immunocytochemistry including species reactivity and working dilutions.

Journal: Autophagy

Article Title: Autophagy mediates the clearance of oligodendroglial SNCA/alpha-synuclein and TPPP/p25A in multiple system atrophy models

doi: 10.1080/15548627.2021.2016256

Figure Lengend Snippet: A table depicting the primary and secondary antibodies used either in Western blot or immunocytochemistry including species reactivity and working dilutions.

Article Snippet: Primary Antibody against /relative reference Clone Catalog number Working dilutions (WB) Working dilutions (ICC) Species Reactivity Company aggregated SNCA [ 40 ] MJFR 14-6-4-2 ab209538 - 1:1000 rabbit Abcam human SNCA [ 87–89 ] LB509 807,701 - 1:1000 mouse Biolegend Syn211 36–008 - 1:2000 mouse Millipore 4B12 1:1000 - mouse GeneTex oxidized/ nitrated SNCA [ 39 ] SYN303 824,301 - 1:1000 mouse Biolegend rodent SNCA D37A6 4179 1:1000 1:400 rabbit Cell Signaling Technology total SNCA C20 sc7011R 1:1000 1:1000 rabbit Santa Cruz Biotechnology SYN1 610,786 - 1:1000 mouse BD Transduction Laboratories TUBA 62,004 - 1:750 mouse Invitrogen ACTB TA309077 1:2000 - mouse OriGene LC3 LC3B PM036 NB100-2220 1:1000 -1:200 rabbit rabbit MBL Novus TPPP/p25A 1:1000 1:400 rabbit/rat SQSTM1/ p62 PM045 1:1000 - rabbit MBL Ubiquitin Z0458 1:1000 - rabbit DAKO Phospho-MTOR D9C2 5536 1:1000 - rabbit Cell Signaling Technology Phospho-ULK D1H4 5869 1:1000 - rabbit Cell Signaling Technology Total ULK D8H5 8054 1:1000 - rabbit Cell Signaling Technology BECN1 H-300 sc-11,427 1:1000 - rabbit Santa Cruz Biotechnology Phospho-RPS6 D57.2.2E 4858 1:1000 - rabbit Cell Signaling Technology Total RPS6 5G10 2217 1:1000 - rabbit Cell Signaling Technology LAMP2A 51–2200 1:1000 1:1000 rabbit Invitrogen HSPA8/HSC70 Ab1427 1:1000 - rabbit abcam ATG5 10,181-2-AP - 1:400 rabbit proteintech Secondary antibody Catalog number Working dilutions (ICC or WB) Species Reactivity Company Goat anti-mouse IgG-HRP conjugated AP124P 1:10,000 mouse Sigma-Aldrich Goat anti-rabbit IgG-HRP conjugated AP132P 1:10,000 rabbit Sigma-Aldrich CF555 red 20,033 1:2000 rabbit Biotium 20,030 1:2000 mouse Biotium 20,096 1:2000 rat Biotium CF488A green 20,012 1:2000 rabbit Biotium 20,010 1:2000 mouse Biotium 115–175-146 1:400 mouse Jackson Imm.

Techniques: Western Blot, Immunocytochemistry, Transduction, Ubiquitin Proteomics, Concentration Assay

( A ) U2OS cells subjected to BAP31 knockout via CRISPR-Cas9 system (sgControl and sgBAP31-2) were subjected to TEM. Higher magnification images are shown at the right. Scale bars, 10 and 1 μm (left and right, respectively). White arrows indicate autolysosomes. ( B ) Total number of autolysosomes in each cell was determined. Data are presented as means ± SD ( n = 6). ( C ) Loss of BAP31 increases LC3-II expression. U2OS cells were transfected with the indicated concentrations of siBAP31 and 150 pmol of siControl for 24 hours. Cells were subjected to immunoblotting using anti-BAP31, anti-LC3, and anti–β-actin antibodies. ( D ) U2OS cells stably expressing GFP-LC3 were transfected with 100 pmol of siBAP31 or siControl for 24 hours. Cells were fixed with 4% paraformaldehyde, and GFP-LC3 (green) fluorescence was determined. Blue represents nuclear 4′,6-diamidino-2-phenylindole (DAPI) staining. Scale bar, 10 μm. The number of LC3-GFP puncta in the cells (green dots) was determined, and data are presented as means ± SD ( n = 6). ( E ) Loss of BAP31 stimulates autophagosome synthesis. U2OS cells were transfected with 100 pmol of siControl or siBAP31 for 24 hours, followed by treatment with or without bafilomycin A1 (1 μg/ml) for 1 hour. Cells were subjected to immunoblotting using the indicated antibodies. ( F ) BAP31 does not affect the ER stress response. U2OS cells were transfected with siBAP31 and siControl for 18 hours and then treated with or without BFA (1 μg/ml) for 8 hours. Cells were subjected to immunoblotting using the indicated antibodies and Phos-tag SDS–polyacrylamide gel electrophoresis (PAGE) or normal SDS-PAGE. ( G ) BAP31 knockout or knockdown activates the AMPK-ULK-LC3 signaling pathway. U2OS and HeLa cells subjected to BAP31 knockout via the CRISPR-Cas9 system (sgControl, sgBAP31-2, and sgBAP31-3) and MEF cells transfected with siControl (200 pmol) and siBAP31 at the indicated concentrations for 24 hours were subjected to immunoblotting using the indicated antibodies. P value was calculated using two-way analysis of variance (ANOVA). ** P < 0.01 (B and D).

Journal: Science Advances

Article Title: BAP31 regulates mitochondrial function via interaction with Tom40 within ER-mitochondria contact sites

doi: 10.1126/sciadv.aaw1386

Figure Lengend Snippet: ( A ) U2OS cells subjected to BAP31 knockout via CRISPR-Cas9 system (sgControl and sgBAP31-2) were subjected to TEM. Higher magnification images are shown at the right. Scale bars, 10 and 1 μm (left and right, respectively). White arrows indicate autolysosomes. ( B ) Total number of autolysosomes in each cell was determined. Data are presented as means ± SD ( n = 6). ( C ) Loss of BAP31 increases LC3-II expression. U2OS cells were transfected with the indicated concentrations of siBAP31 and 150 pmol of siControl for 24 hours. Cells were subjected to immunoblotting using anti-BAP31, anti-LC3, and anti–β-actin antibodies. ( D ) U2OS cells stably expressing GFP-LC3 were transfected with 100 pmol of siBAP31 or siControl for 24 hours. Cells were fixed with 4% paraformaldehyde, and GFP-LC3 (green) fluorescence was determined. Blue represents nuclear 4′,6-diamidino-2-phenylindole (DAPI) staining. Scale bar, 10 μm. The number of LC3-GFP puncta in the cells (green dots) was determined, and data are presented as means ± SD ( n = 6). ( E ) Loss of BAP31 stimulates autophagosome synthesis. U2OS cells were transfected with 100 pmol of siControl or siBAP31 for 24 hours, followed by treatment with or without bafilomycin A1 (1 μg/ml) for 1 hour. Cells were subjected to immunoblotting using the indicated antibodies. ( F ) BAP31 does not affect the ER stress response. U2OS cells were transfected with siBAP31 and siControl for 18 hours and then treated with or without BFA (1 μg/ml) for 8 hours. Cells were subjected to immunoblotting using the indicated antibodies and Phos-tag SDS–polyacrylamide gel electrophoresis (PAGE) or normal SDS-PAGE. ( G ) BAP31 knockout or knockdown activates the AMPK-ULK-LC3 signaling pathway. U2OS and HeLa cells subjected to BAP31 knockout via the CRISPR-Cas9 system (sgControl, sgBAP31-2, and sgBAP31-3) and MEF cells transfected with siControl (200 pmol) and siBAP31 at the indicated concentrations for 24 hours were subjected to immunoblotting using the indicated antibodies. P value was calculated using two-way analysis of variance (ANOVA). ** P < 0.01 (B and D).

Article Snippet: Antibodies used for immunoblotting were specific for the following proteins: LC3b, PERK, IRE1α, P-AMPK, AMPK (total), P-ULK (Ser 757 ), P-ULK (Ser 317 ), ULK (total), COXIV, cytochrome c, calnexin, HSP60, mitofusin-1, and Bcl-2 (Cell Signaling Technology); BAP31, ATF6, Tom40, Tom22, prohibitin, and VDAC1 (Santa Cruz Biotechnology); Parkin and FACL-4 (Abcam); and NDUFS4, NDUFB11, α-tubulin, β-actin, and flag (Sigma-Aldrich).

Techniques: Knock-Out, CRISPR, Expressing, Transfection, Western Blot, Stable Transfection, Fluorescence, Staining, Polyacrylamide Gel Electrophoresis, SDS Page, Knockdown